Fig. 1

Multiomics data analysis in the study cohort. Panel A shows 16S sequencing result on 12 joint-fluid DNAs. The x-axis shows samples and y-axis shows number of 16s sequencing reads. Detected bacterial species is shown on the top of each bar. Panel B shows a heatmap on 48-plex cytokine expression in all 12 joint-fluid specimens. Blue and red color of the heatmap indicate relatively low and high cytokine expression, respectively. The blue bar-plot underneath heatmap shows sum of cytokine responses in each sample. Panel C shows a heatmap on IgG antibody protein array data. Each sample presents joint-fluid IgG antibody reactivities against 120 autoantigens labeled on the side axis. Blue and red color of the heatmap indicate relatively low and high autoantibody level, respectively. The pink bar-plot underneath heatmap shows sum of autoantibody responses in each sample. Some clinically significant autoantigens are listed in boxes on the side. Panel D presents single cell RNA sequencing data-based t-SNE plot on JF7, JF8 and JF12 sample. Each dot represents individual single cell. Detected immune cell types are indicated with different colors