Fig. 2
Single cell RNA sequencing unravel immune cell composition in infection negative and infection positive joint-fluids. Panel A shows t-SNE plot on various immune cell clusters detected in one infection positive (JF7) and two infection negative and autoantibody positive subjects (JF8 & JF12). Various cell clusters are generated based on the cell-specific gene expression signatures. Neutrophils are indicated with blue color, T cells with pink (CD8) and orange (CD4) and B cells with red color. Other small cell types are indicated by other colors. Each dot represent one individual cell detected by 10X genomics cell-ranger data analysis pipeline. Panel B shows distribution and abundance of central memory CD8+ T cells in three samples. Panel C presents percentage of CD8+CCR7+ autoreactive T cells in three samples. Panel D presents percentage of CD4+CXCR5+ Tfh cells in three samples. Single cell gene expression matrix was generated using 10X Genomics’s Cell Ranger pipeline. Various cell clusters are visualized using 10x Genomics’s Loupe browser software