Table 1 Demographics, clinical chracteristics and laboratory test results of the study cohort
Case ID | Demographic data | Standard of care blood tests | Standard of care Joint fluid tests | mNGS | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Sex | Age | Race | WBC count (thousands/mm3) | Absolute neutrophil count (thousands/mm3) | Platelet count (thousands/mm3) | Initial CRP | Initial ESR | Initial PCT | Peak CRP | Nucleated cells in JF (cells/mm3) | Absolute neutrophil count in JF (cells/mm3) | Standard culture | Standard of care PCR | Total 16S sequencing read counts in JF | 16S Identified potential pathogenic species | |
(mg/L) | (mm/h) | (ng/mL) | (mg/L) | |||||||||||||
JF-1 | Male | 7 years | Hispanic | 15.5 | 12.2 | 475 | 263 | > 120 | 0.39 | 263 | 14,650 | 12,452 | Negative | Streptococcus Pyogenes | 55,526 | Streptococcus Pyogenes |
JF-2 | Female | 2 years | Hispanic | 23.6 | 18.6 | 538 | 82 | 92 | 0.41 | 82 | 222,500 | 198,025 | Streptococcus Pyogenes | Streptococcus Pyogenes | 31,505 | Streptococcus Pyogenes |
JF-3 | Male | 3 months | White | 15.1 | 4.8 | 726 | 59 | 35 | 0.11 | 64 | 155,000 | 141,050 | Staphylococcus aureus | Negative | 4,232 | Staphylococcus aureus |
JF-4 | Female | 2 years | Hispanic | 9.2 | 4.8 | 188 | 100 | 38 | 0.94 | 100 | Not tested | Not tested | Streptococcus Pyogenes | Streptococcus Pyogenes | 26,366 | Streptococcus Pyogenes |
JF-5 | Male | 3 years | White | 5.4 | 1.5 | 373 | 4 | 13 | < 0.04 | Not tested | 6,750 | 5,130 | Negative | Negative | 3,093 | Negative |
JF-6 | Male | 13 months | White | 18.6 | 5.9 | 519 | 125 | 40 | 0.06 | 125 | 65,650 | 64,337 | Negative | Negative | 904 | Negative |
JF-7 | Female | 7 years | Hispanic | 21.6 | 18.1 | 244 | 159 | 45 | 1.05 | 316 | 119,000 | 102,340 | Streptococcus Pyogenes | Streptococcus Pyogenes | 23,935 | Streptococcus Pyogenes |
JF-8 | Female | 17 months | Hispanic | 12.6 | 2.9 | 674 | 21 | 63 | 0.09 | Not tested | Not tested | Not tested | Negative | Negative | 5,564 | Negative |
JF-9 | Male | 2 years | Hispanic | 9.5 | 4.9 | 310 | 27 | 43 | 0.07 | Not tested | 93,375 | 78,435 | Negative | Negative | 548 | Negative |
JF-10 | Male | 12 years | White | 14.3 | 12.4 | 79 | 268 | 37 | 7.89 | 268 | Not tested | Not tested | Staphylococcus aureus | Not tested | 51,010 | Staphylococcus aureus |
JF-11 | Male | 11 years | White | 7.6 | 5.2 | 210 | 280 | 45 | 0.12 | 280 | 34,625 | 29,431 | Negative | Negative | 2,240 | Negative |
JF-12 | Female | 3 years | Black | 7.7 | 4.7 | 379 | 18 | 70 | 0.04 | Not tested | 20,563 | 18,712 | Negative | Negative | 322 | Negative |