Fig. 3
From: T-ing up the storm: pathogenic cycling lymphocytes in the biology of macrophage activation syndrome
Identification and quantification of CD38+HLA-DR+T cells by flow cytometry. (A) Required and optional antibodies for the detection of CD38+HLA-DR+ T cells by flow cytometry. Optional antibodies are used to exclude other leukocyte subsets including NK cells (CD56), monocytes (CD14), neutrophils (CD16) and B lymphocytes (CD19). (B) Illustration of gating strategy for CD38+HLA-DR+ T lymphocytes and typical results from a healthy individual. The percentage of positive cells is shown in boxes. (C) Example of CD38+HLA-DR+ T cell quantification performed serially in a patient with Still’s disease complicated by MAS